QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Generation of Akhr mutants. (A) Schematic representation of Akhr alleles. Akhr^p (EY11371) contains a P element inserted in exon 1, upstream of the start of translation. Akhr^rev is a P-element excision allele; an 20 bp remnant of the P element remains. Akhr^null removes the entire coding sequence of the Akhr gene, but does not disrupt the adjacent CG11188 and Tsp genes. The Akhr^null line also retains an insert of the P element (400 bp) and removes 150 bp of genomic sequence downstream of Akhr. See the Materials and methods section for further details. (B) Akhr mRNA is expressed in the fat body of Akhr^rev (left lane for each primer pair) but not in Akhr^null (right lane for each primer pair) as assessed using RT-PCR. Akhr mRNA was also detected in y¹w^67c23 and Akhr^p (EY11371) lines (data not shown). All bands are of the expected molecular mass; see the Materials and methods section for a description of the PCR protocol. (C) Transgenic flies that expressed the yeast transcriptional activator Gal4 under the control of the Akhr promoter (Akhr-Gal4) were crossed to reporter flies (UAS-GFP). Expression in the fat body (white arrows) was observed throughout the adult body, including head (D) and dissected fat body tissue (E); expression in the larval fat body was also observed (data not shown).

Return to article