Files in this Data Supplement:

• Movie S1 -

  Movie 1. Two worker honey bees (Apis mellifera), aroused from sleep within observation hive. The workers are in a sleep state (relatively immobile in resting posture and discontinuously ventilating) until they are sequentially perturbed by an oncoming sibling. Contact made by hive mates often results in arousal, which is typically followed by grooming.

• Movie S2 -

  Movie 2. Worker honey bees (Apis mellifera) exhibiting the deepest state of sleep. Honey bees that sleep with motionless antennae experience the highest arousal thresholds. Antennal immobility is accompanied by discontinuous ventilation in sleeping bees. Toward the end of the video, the centrally located bee’s gaster shakes in a short burst of respiratory pumping.

• Movie S3 -

  Movie 3. This worker honey bee (Apis mellifera) is in a sleep state, but instead of her antennae being motionless, they are slightly twitching and she presumably experiences a higher arousal threshold.

• Movie S4 -

  Movie 4. A worker honey bee (Apis mellifera) is in a sleep state considered ‘transitional’ by Kaiser (Kaiser, 1988) if the arousal threshold is lower and the bee exhibits spontaneous antennal movements. One antenna of this worker is making movements larger than the twitches of the bee in Movie 3.