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Fig. 1. The microfluidic device. (A) Microfluidic device and portion of connected
tubing, shown with a dime for scale (17.91 mm in diameter). (B) Drawing of
microchannels in the device. (C) Micrograph of test region, showing flow
channels and barrier. Solid arrows indicate direction of flow in the device
during its normal operation. Dashed arrow shows flow in channel 5 during the
removal of cells from the barrier. Dashed lines mark the boundaries between
streams from channels 2, 1 and 3. (D) Schematic three-dimensional drawing of
barrier and cells (not to scale). Curved dashed lines are flow lines with
arrows indicating flow direction. B and C are rotated 90 deg. counterclockwise
with respect to A and D, as indicated by the orientation of the x-
and y-axis shown in the panels.
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