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Fig. 3. Hemolymph levels of MF increase after eyestalk ablation (ESA). Animals
without detectable methyl farnesoate (MF) were held in 33 ppt SW at 11°C
until used. (A) All of the green-phase (N=6) and red-phase
(N=5) males had detectable MF after ESA. On day 1, the amount of MF
(values ± s.e.m.) was lower in green-phase males than in red-phase
crabs (***P<0.001, t-test). By day 5 the
levels were similar (P>0.05, t-test). (B) Red-phase
(N=8) and (C) green-phase (N=7) males were treated with 5 or
33 ppt SW at 11 or 18°C, 5 or more days after ESA. SW at 5 ppt did not
significantly increase MF levels of crabs in either color phase at either
temperature. SW at 18°C increased MF levels, most notably at 5 ppt (bars
with different letters = P<0.01, ns = P>0.05;
ANOVA).
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