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Figure 4


Fig. 4. O2-dependent H2S production and consumption measured in real-time with a polarographic H2S sensor. (A,B) H2S production (as total sulfide) by minced trout heart increases with increasing concentrations of cysteine (mmol l–1) at PO2~0 and is transiently inhibited by injection of oxygen (O2) into the reaction chamber. H2S production resumes, presumably after the oxygen has been depleted (post O2). (A) A single representative trace, (B) pooled results expressed as means ± s.e.m. (N=4). (C,D) H2S is consumed by addition of purified trout heart mitochondria (M). The rate of sulfide consumption is greater when the reaction mixture is equilibrated with room air (O2) than with nitrogen (N2) or if the mitochondria are heat denatured (Heat). w, wash. (C) Single representative trace, (D) pooled results expressed as means ± s.e.m. (N=4 replicates). A, B adapted from Whitfield et al. (Whitfield et al., 2008), with permission; C, D adapted from Olson et al. (Olson et al., 2008b), with permission.





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