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Figure 2


Fig. 2. Denaturing Tricine-SDS-PAGE of the foam fluid and fractions obtained during isolation of the surfactant 20 kDa protein. Lane 1, molecular mass standards; lane 2, foam fluid (20 µg); lanes 3 and 4, non-retained and retained fractions, respectively, in CM-Sepharose (20 µg). Molecular mass markers were phosphorylase b (97.0 kDa), bovine serum albumin (67.0 kDa), egg white albumin (45.0 kDa), carbonic anhydrase (29.0 kDa), soybean trypsin inhibitor (20.1 kDa) and {alpha}-lactalbumin (14.2 kDa).





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