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Fig. 10. Cryo-SEM micrographs of the leg cuticle of different insects. (A–F)
Adult Pameridea roridulae bug. Cross-fractures of (A) a freshly
killed and (B) a dead, dry mirid bug show a distinct layer of epicuticular
grease. (C) The layer disappears after washing bugs in cold chloroform. Top
views of cuticle in (D) a freshly killed, (E) a dead, dry and (F) a dry,
chloroform-washed mirid bug. (G–I) Adult Calliphora vicina fly.
Cross-fractures of (G) a freshly killed, (H) dead, dry and (I) a dry,
chloroform-washed C. vicina. All preparations are missing a distinct
epicuticular grease layer. The arrowheads indicate epicuticular grease layer;
m, microtrichia; ep, epicuticle; ex, exocuticle; en, endocuticle. Scale bars,
2 µm.