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Fig. 2. Anatomical features of IV neurons. (A) C. pagurus brain with
stained IV neurons after backfilling the ivn with NiCl2.
The areas indicated by the black boxes are magnified below the main image, and
bottom right shows the somata of the IV neurons in area 17 [nomenclature after
Sandeman et al. (Sandeman et al.,
1992)]. No arborizations within the brain were observed. (B)
Frontal section of a brain showing the dorso-medial location of the IV neuron
somata. Somata were stained with a Lucifer Yellow backfill of the
ivn. (C) Cross-section of the ivn. Eight axons were visible
(arrows), two of which belonged to the IV neurons. (D) Schematic drawing of
ivn projections in the brain, as revealed by ivn backfills.
The somata of the IV neurons marked by the arrow were located in area 17 of
the brain. Two (ascending) axons projected along the midline towards the
anterior part of the brain, but could not be followed further than the AMPN
(anterior medial protocerebral neuropil). The four other axons projected
towards the coc. (E) Schematic drawing of IV neuron projections
(summary). The IV neurons project via the son and the
ion to the CoG and via the stn to the STG.