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Figure 8


Fig. 8. Quintuple-color immunofluorescence staining for Na+/K+-ATPase (red), NKCC1a (blue), NCC (cyan), NHE3 (yellow) and CFTR (green), at (A–F) 0h and (G–L) 72h of the freshwater-to-seawater transfer experiment, and at (M–R) 0h and (S–X) 72h of the seawater-to-freshwater transfer experiment. Arrowheads, arrows and double-lined arrows indicate the apical immunoreactivity for NCC, NHE3 and CFTR, respectively. I, type-I mitochondria-rich cell (MRC); II, type-II MRC; III, type-III MRC; IV, type-IV MRC. Scale bar, 10 µm.





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