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Fig. 2. Multiple alignment of the deduced amino acid sequence of tilapia NKCC1a
(GenBank accession no. AY513737), tilapia NKCC1b (AY513738), tilapia NKCC2
(AY513739) and tilapia NCC (EU518934) cDNAs that were isolated from the gills
of Mozambique tilapia. Conserved and similar amino acids are shaded in black
and gray, respectively. Putative transmembrane segments are underlined
(TM1–TM12). The boxed amino-terminal sequences of tilapia NKCC1a and
tilapia NCC were used as antigens to produce antibodies against tilapia NKCC1a
and tilapia NCC.
