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Figure 4


Fig. 4 (A) The percentage of cells (± s.e.m.) from cell monolayers of Tiedemann body (TB), coelomic epithelium (CE), axial organ (AO), pyloric caeca (PC) and coelomocytes (CC) that show FITC-labelled anti-BrdU during a time course (4, 16 and 24h) post-injection of LPS or CMFSS buffer (N=4). The numbers of labelled cells indicate the rate of cell division during 3h of BrdU incubation, as described in Materials and methods. Proliferation in pyloric caeca and coelomocytes was not affected, but in the Tiedemann body, coelomic epithelium and axial organ, proliferation had already increased at 4h. The corresponding organs of the animals pre-treated with CMFSS (control) also showed an increase in proliferation but the response came later. (B) Table presenting the differences between the mean values of proliferation and the P-values when comparing the initial proliferation of the different organs of LPS-treated and Control animals and between the results obtained at 4 and 24h p.i.





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