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Fig. 8. Effect of HOE642, ouabain, catalase, SOD, SP600125 (SP) and AS601245 (AS)
on hyperthermia-induced cleavage of PARP (A: upper panel). Proteolytical
processing of PARP was detected in extracts (50 µg of protein) from control
hearts (0.5 h equilibration followed by 4 h perfusion at 25°C) or hearts
perfused after equilibration for 4 h at 42°C in the presence or absence of
HOE642 (5 µmol l–1), ouab (100 µmol
l–1), catalase (150 U ml–1), SOD (30 U
ml–1), SP (10 µmol l–1) and AS (1 µmol
l–1). To confirm equal protein loading the membranes were
re-probed with an anti-actin antibody (A: bottom panel). Densitometric
analysis of the band corresponding to the fragment of PARP was performed by
laser scanning (B). Western blots shown are representative of at least three
independent experiments while data are means ± s.e.m. for at least
three independent experiments. 
P<0.001
vs control hearts and *P<0.001 vs
hearts perfused at 42°C.
