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Fig. 6. Effect of HOE642, ouabain, catalase and SOD on hyperthermia-induced
phosphorylation of JNKs (A: upper panel). Phosphorylated JNKs were detected in
extracts (50 µg of protein) from control hearts (0.5 h equilibration
followed by 1 h perfusion at 25°C) or hearts perfused after equilibration
for 1 h at 42°C in the presence or absence of HOE642 (5 µmol
l–1), ouabain (100 µmol l–1), catalase
(150 U ml–1) and SOD (30Uml–1). The effects
of HOE642 and ouabain alone were also assessed. As a control for equal protein
loading, an anti-actin antibody was used (A: bottom panel). Densitometric
analysis of phospho-JNKs (B) was performed by laser scanning. (C) Relationship
of the net JNK phosphorylation level (%) induced by hyperthermia in the
presence of the agents assessed to the hyperthermia-induced JNKs
phosphorylation level. Western blots shown are representative of at least
three independent experiments while data are means ± s.e.m. for at
least three independent experiments. 
P<0.001
vs control hearts and *P<0.01 vs
hearts perfused at 42°C.
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