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Fig. 5. Effect of HOE642 (HOE), ouabain (Ouab.), catalase (Cat.) and superoxide
dismutase (SOD) on hyperthermia-induced phosphorylation of p38-MAPK (A: upper
panel). The phosphorylated form of the kinase was detected in extracts (50
µg of protein) from control hearts (0.5 h equilibration followed by 1 h
perfusion at 25°C) or hearts perfused after equilibration for 1 h at
42°C in the presence or absence of HOE642 (5 µmol
l–1), ouab (100 µmol l–1), catalase (150
U ml–1) and SOD (30 U ml–1). The effects of
HOE642 and ouabain alone were also assessed. As a control for equal protein
loading an anti-actin antibody was used (A: bottom panel). Densitometric
analysis of phospho-p38-MAPK (B) was performed by laser scanning. (C)
Relationship of the net p38-MAPK phosphorylation levels induced by
hyperthermia in the presence of the agents assessed to the
hyperthermia-induced p38-MAPK phosphorylation levels. Western blots shown are
representative of at least three independent experiments while data are means
± s.e.m. for at least three independent experiments.
**P<0.001 vs control hearts and
P<0.001 vs hearts perfused at
42°C.
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