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Fig. 4. Profile of caspase 3 and poly(ADP-ribose) polymerase (PARP) cleavage in
samples from isolated Rana ridibunda hearts perfused after
equilibration at 42°C for increasing periods of time as indicated. (A)
Upper panel: pro-caspase 3 immunoreactivity was observed with no cleaved
active forms of caspase 3 detected. (B) Upper panel: proteolytic processing of
PARP was observed and quantification of its fragment was performed by laser
scanning densitometry (C). The membranes were re-probed with an anti-actin
antibody so as to verify equal protein loading (A,B: bottom panels). Western
blots shown are representative of at least three independent experiments while
data are means ± s.e.m. for at least three independent experiments.
*P<0.05 and 
P<0.001
vs control hearts (0.5 h equilibration followed by 1 h perfusion at
25°C).
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