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Figure 5


Fig. 5. Detection of NHE2-expressing cells in rainbow trout gill by combined immunofluorescence and in situ hybridization. (A) PNA+ MRCs (labelled with arrows) were detected using a primary antibody against Na+/K+-ATPase (red) and biotinylated PNA (green). (B) In situ hybridization on the same section demonstrated that the PNA+ MRCs were enriched with NHE2 mRNA (labelled with arrows). Two PNA+ cells (labelled with asterisks) did not exhibit strong mRNA staining. No staining was observed when a sense probe was used (C) or when probe was omitted (D). Scale bar, 20 µm.





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