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Fig. 4. Identification of NHE3-expressing cells as PNA+ MRCs in gill
sections from adult rainbow trout. (A) NHE3 protein was detected in gill (G)
and kidney (K) protein extracts using Western blots (N=6) to reveal a
98 kDa immunoreactive band (lanes 1 and 2) that was absent after the NHE3
antibody was incubated with excess peptide against which the antibody was
raised (lanes 3 and 4). Size markers are given in kDa. (B) Co-localization of
NHE3 (green) and Na+/K+-ATPase (red) in MRCs (nuclei are
stained blue); NHE3-expressing MRCs are labelled with arrows and NHE3 negative
MRCs are indicated by stars. (C) Localization of NHE3 to PNA+ MRCs;
red indicates NHE3, green indicates PNA and nuclei are stained blue. (D) The
NHE3 immunofluorescence disappeared with omission of primary NHE3 antibody.
Scale bar in A, 40 µm; and in C, 10 µm.
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