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Figure 3


Fig. 3. (A) Identification of PNA+ and PNA– negative mitochondria-rich cells (MRCs) in gill sections from adult rainbow trout. PNA+ MRCs are labelled with arrows; stars indicate PNA– MRCs. MRCs were identified on the basis of immunoreactivity against Na+/K+-ATPase (red); green indicates apical PNA and blue indicates nuclei (DAPI). (B) Higher magnification image of a PNA (green)-positive MRC. Negative controls included (C) omission of primary antibody against Na+/K+-ATPase and of biotinylated PNA. The image in C was captured using the same exposure and microscope settings as in A. Scale bar in A, 20 µm; and in B, 5 µm.





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