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Fig. 1. (A) Phylogenetic relationship of selected NHE1–3 proteins from
different species and orthologous rainbow trout NHE2, NHE3a and NHE3b amino
acid sequences. The grouping of each rainbow trout orthologue with its
corresponding form is an indication that the rainbow trout clone is most
closely related to that NHE isoform. (B) The existence of the two rainbow
trout NHE3 transcripts is indicated in a representative Northern blot.
K1–6, kidney samples 1–6. The tree in A was constructed using the
neighbour-joining method, and numbers indicate bootstrap values for 1000
replicates. GenBank accession numbers are as follows. NHE1: Oncorhynchus
mykiss (βNHE) Q01345, Pseudopleuronectes americanus
AAO32340, Amphiuma tridactylum AAD33928, Takifugu obscurus
BAE75800.1, Rattus norvegicus NP_036784.1, Anguilla anguilla
CAB45085, Cyprinus carpio CAB45232, Homo sapiens NP_003038,
Drosophila melanogaster AAF51559, Gallus gallus ABB82239.
NHE2: Canis familiaris XP_531775.2, Gallus gallus
XP_416918.2, Mus musculus NP_001028461 XP_129721, Oryctolagus
cuniculus P50482, 708 amino acid (aa) Danio rerio
XP_001336127.1, 795 aa Danio rerio XP_691364, Homo sapiens
NP_003039, Myoxocephalus octodecemspinosus AAD46576, Rattus
norvegicus P48763, partial 692 aa Oncorhynchus mykiss ABO32814,
Squalus acanthias ABC54565, Tetraodon nigroviridis CAG05798.
NHE3: 704 aa Danio rerio CAM47009.1, 829 aa Danio rerio
XP_696670, 851 aa Danio rerio XP_696728, Gallus gallus
XP_418895.2, Mus musculus NP_001074529.1, Dasyatis sabina
AAT45738.2, Homo sapiens NP_004165.1, Rattus norvegicus
NP_036786.1, NHE3a; 752 aa Oncorhynchus mykiss ABO32815.1 and NHE3b;
862 aa Oncorhynchus mykiss assembled sequence, Oreochromis
mossambicus BAF80347, Tribolodon hakonensis AB055466.
