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Figure 2


Fig. 2. Expression of genes involved in ion secretion increased in both gills (A) and opercular epithelium (B) after seawater transfer. Na+/K+-ATPase {alpha}1a subunit, Na+/K+/2Cl– cotransporter 1 (NKCC1), cystic fibrosis transmembrane conductance regulator (CFTR) Cl– channel, Na+/H+ exchanger isoform 3 (NHE3) and carbonic anhydrase isoform II (CAII) mRNA levels are shown after transfer from brackish water (10% seawater) to brackish water (BW; handling control, grey bars) or seawater (SW; black bars). Expression is relative to the expression of EF1{alpha} and is normalized to pre-transfer brackish water controls. Data are expressed as means ± s.e.m. (N shown within each bar). *Significantly different from time-matched brackish water controls (P<=0.05); {dagger}significantly different from pre-transfer (Pre) brackish water controls (assessed using a post-hoc test; P<=0.05).





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