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Figure 2


Fig. 2. Calcium imaging of isolated crypt olfactory receptor neurons (ORNs). In each panel the upper row of images shows total fluorescence; the lower row shows a bright-field image, maximum relative fluorescence increase ({Delta}F/F) and an overlay of both. The corresponding plots display the time course of the normalized fluorescence average of the whole crypt ORN and its supporting cell. Amino acids (A), IBMX plus forskolin (B) and 8Br-cAMP (C) induced a transient rise of intracellular calcium in the crypt ORN. Some supporting cells, which are coupled to the crypt ORN by gap junctions (Schmachtenberg, 2006), were included in the calcium signal (B,C) whereas others were apparently excluded (D). The effects were similar in amplitude and duration to those of stimulation with KCl (50 mmol l–1 in the stimulus pipette). Bile salts (E) and the combination of PGF2{alpha} and 17,20βP (F) did not cause significant and reproducible effects. bf, bright-field image; co, crypt ORN; sc, supporting cell.





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