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Fig. 2. Ethological characterization of sleep stages in honeybees. (A) Bout
duration at different sleep stages (mean ± s.e.m.), for a
representative forager. Similar results were obtained for 15 additional
foragers (N=21–194 bouts/bee). (B) Antennae movements at
different sleep stages (mean ± s.e.m.), for a representative forager.
Similar results were obtained for 16 additional foragers
(N=131–906 min/bee). Different capital letters indicate
statistically significant differences. (C) Group summary of antennae movement
data for all foragers and callows. There was no significant difference between
foragers and callows (see supplementary material Table S1). (D) Summary of
bout duration data for all foragers and callows. Bout duration differed
between foragers and callows (see supplementary material Table S2). Numbers
within or above bars are the sample sizes. Filled bars, foragers; open bars,
callows; left panels, colony H3; middle panels, colony H12; right panels,
colony S25. FS, first sleep stage; SS, second sleep stage; TS, third sleep
stage.