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Fig. 3. (A) Diagram of the reproductive system of Aplysia showing the
sites tested for localization of the effect of NO. (B) Analysis of the effect
of the NO donor application to the abdominal ganglion. (i) Effect of
application of the NO donor on the activity recorded extracellularly using a
suction electrode placed on the cut pleural-abdominal connective, close to the
bag cells. The two traces are continuous. The stimulus did not elicit a bag
cell after-discharge. (ii) Electrical stimulation of the connective (in the
example shown, 7 V, 7 Hz, 5 ms pulse duration, maintained for 5 s) induced an
after-discharge. The three traces are continuous. The recordings began a few
seconds after the stimulus, since the same suction electrode was used for
stimulation and recording. The stimulating electrode was removed from the
tubing and the recording electrode was then inserted.
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