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Fig. 6. (A) Mitochondria-rich cells and associated innervation in the gills of
zebrafish. Mitochondria-rich cells (MRCs; green) of the interlamellar regions
were immunoreactive for antibodies against the 
5 subunit of
Na+/K+-ATPase, and nerve fibres (red) were labelled with
the neuronal marker zn-12. The confocal image illustrates the association of
MRCs (arrows) of the gill filaments (F) with nerve fibres (arrowheads indicate
apparent points of contact). Scale bar, 10 µm. Reprinted with permission
(Jonz and Nurse, 2006). (B)
Innervation of the pseudobranch. Methods similar to those used in A were used
to label 5-positive pseudobranch cells (PBCs; green) and zn-12-positive
nerve fibres (red) in zebrafish. Larger nerve bundles (arrows) of the
supportive filaments gave rise to a network of nerve fibres (arrowheads) that
ramify among the fused lamellae and innervated PBCs. Scale bar, 20 µm.
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