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Fig. 5. Confocal imaging of neuroepithelial cells (NECs) and associated innervation
of the gill filaments and lamellae in zebrafish. NECs were labelled with
antibodies against 5-hydroxytryptamine (5-HT; green) and SV2 (red in A; red or
yellow in B), and nerve fibres were identified by zn-12 immunoreactivity
(red). (A) SV2-positive NECs (arrows) were found in the filament (F) and
received innervation from intrinsic nerve fibres (iNF) and a plexus of
extrinsic nerve fibres (eNF). Extrinsic nerve fibres were also found in the
lamellae (L). Scale bar, 20 µm. (B) Dual exposure image in A showing, in
addition, 5-HT-positive immunofluorescence (green) and that some NECs
containing SV2 are also serotonergic.
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