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Figure 4


Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo-β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo-β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.





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