|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 13. Carbohydrates produced by the hydrolysis of laminarin when incubated with
laminarinase from G. natalis (A) and C. destructor (B), and
by the hydrolysis of carboxymethyl cellulose when incubated with
endo-β-1,4-glucanase purified from G. natalis (C) and C.
destructor (D). Hydrolysate and standards (glucose and cellobiose) were
run on a silica thin layer chromatography (TLC) plate with a mobile phase of
n-butanol/acetic acid/water (2:1:1, v/v/v)
(Nishida et al., 2007).
Carbohydrate products were visualised by spraying the TLC plate with 10% (v/v)
sulphuric acid in ethanol and incubating at 120°C for 15 min
(Nishida et al., 2007).
|
