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Fig. 10. Gel filtration chromatography of endo-β-1,4-glucanases derived from
the midgut gland of C. destructor. Samples were loaded onto a Bio-Rad
P-100 gel filtration column and the protein concentration (mg protein
ml–1, 
) and endo-β-1,4-glucanase activity (µmol
reducing sugars produced min–1 ml–1,
) measured. (A) Chromatography of combined and concentrated fractions
1–6 from DEAE anion exchange chromatography described in
Fig. 5 containing
endo-β-1,4-glucanase 1. (B) Size exclusion chromatography of combined and
concentrated fractions 16–22 from DEAE chromatography described in
Fig. 5 containing
endo-β-1,4-glucanase 2.
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