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Fig. 1. Experimental set-up for in vivo experiments at in situ
pressure (26 MPa) (modified from Ravaux et
al., 2003). (A) The pressure vessel IPOCAMP (Incubateur
Pressurisé pour l'Observation et la Culture d'Animaux Marins Profonds;
internal diameter 20 cm, height 60 cm) contains two PVC cages (length 6 cm,
width 5 cm, height 6 cm) closed at the top with a transparent polyethylene
lid. Three sapphire windows in the pressure vessel lid allow the insertion of
an endoscope and two optical-fibre light-guides, for the observation of the
inside of the cages. Large arrows indicate the inlet and outlet of circulating
seawater, which is forced through the cage ventilation holes via a
PVC tube. Two Pt100 probes are positioned in the water flow indicating the
upstream (T1) and the downstream (T2) temperature. (B) Temperature profiles
for a typical heat shock (HS) experiment (solid line, T1 probe; broken line,
T2 probe; bold line, mean temperature obtained from T1 and T2 probes). The
animals were maintained at 15°C and subsequently exposed to a sharp heat
shock at 30–33°C. After rapid cooling to 15°C, the animals were
recovered at different times after the heat shock (see Materials and methods
for more details). (C) Video view of a cage containing eight P.
grasslei specimens, maintained at a temperature of ca. 30°C
during a HS experiment. The approximate length of P. grasslei
specimens was 7 cm.
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