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Figure 7


Fig. 7. Genomic Southern blot analysis of the MyoD, myogenin, myf5 and MRF4 genes. Genomic DNA prepared from S. macrurus adults was digested with BamHI (lane 1), EcoRI (lane 2), HindIII (lane 3), BamHI/EcoRI (lane 4), BamHI/HindIII (lane 5) and EcoRI/HindIII (lane 6), resolved by 1% agarose gel electrophoresis, and transferred to a nylon membrane. Genomic DNA was loaded at 5 µg per lane. Blots were hybridized with 32P-labeled probes specific to S. macrurus MyoD, myogenin, myf5 and MRF4, and washed under high stringency conditions.





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