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Fig. 6. Expression of Id1, Id2 and MEF2C in adult and 5
week ST muscle and EO by RT-PCR. Agarose gel showing the expression of
Id1, Id2 and MEF2C in skeletal muscle and EO from
control un-operated and 5 week ST fish using RT-PCR. Id1,
Id2 and MEF2C transcripts were detected in all tissues
analyzed. The size of the cDNA fragments for each transcript was the same as
that in Fig. 5; 1 µg of cDNA
was used for PCR amplification. RT-PCR analysis of GAPDH was used as a loading
control. Lanes labeled M represent the 1 kb+ or 25 bp
(Id2) DNA ladder. Other lanes as in
Fig. 3.
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