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Fig. 3. Wing-beat frequency (WBF; s–1) of D. melanogaster
as a function of flight temperature (Ttest, °C),
developmental temperature (Tdev, °C), body mass (A)
and wing area (B). As test temperature increased, wing-beat frequency
significantly increased (plots from left to right). Flies developing at cold
temperatures (blue triangles, 15°C) had significantly lower wing-beat
frequencies at every test temperature compared with flies developing at
intermediate temperatures (orange circles, 23°C) or warmer temperatures
(red squares, 28°C). Males (open symbols) and females (filled symbols) did
not have significantly different WBFs after controlling for wing area and body
size. Heavier flies tended to have faster WBFs (A;
Table 2D), and flies with
larger wings had slower WBFs (B; Table
2D) after statistically controlling for Tdev
and Ttest.