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Figure 4


Fig. 4. Effects of organic osmolytes or colchicine on Ostf mRNA expression levels in primary gill cell culture. Primary gill cells were grown overnight in the isotonic medium (320 mOsmol l–1) and were then subjected to hypertonic treatment (NaCl, 500 mOsmol l–1) with the addition of (A) either 5 mmol l–1 of inositol, betaine or taurine or (B) 100 µmol l–1 colchicine. After 6 h of treatment, total RNA was extracted and was reverse transcribed for real-time PCR assay. Note that the cotreatment of the hypertonicity-exposed cells with the organic osmolytes or colchicine abolished the induced Ostf mRNA expression. *P<0.05 compared with the control cells. The results were obtained from five independent experiments.





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