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Figure 3


Fig. 3. Induction of Ostf mRNA expression levels in primary gill cell culture. Primary gill cells were grown overnight in isotonic medium (320 mOsmol l–1) and were then subjected to hypertonic treatments and/or actinomycin D (Act D) treatment. After 6 h (0.25 days) of treatment, total RNA was extracted and reverse transcribed for real-time PCR assay. (A) Hypertonicity was achieved by the addition of either (1) membrane-nonpermeable solute (NaCl) or (2) membrane-permeable solute (urea) to the medium, making its osmolarity 500 mOsmol l–1. A significant induction of Ostf mRNA expression was observed in the hypertonic condition prepared by the addition of NaCl. (B) Effect of Act D on hypertonicity-induced Ostf mRNA expression. Hypertonicity and Act D cotreatment abolished the induced Ostf transcript levels. *P<0.05 compared with the control cells. The results were obtained from five independent experiments.





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