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Figure 6


Fig. 6. Regulation of UCP2/3 gene expression in amphibians in response to cold and food deprivation. 5 µg total RNA were hybridized with homologous Xenopus laevis UCP2/3 probes. (A) Northern blot analysis showing tissue-specific expression of UCP2/3 in X. laevis. (B) UCP2/3 mRNA expression levels in liver and skeletal muscle of B. marinus in response to cold and fasting. In the liver, cold caused a significant upregulation of UCP2/3 mRNA expression levels while fasting had no effect. In skeletal muscle, no effect of acclimation temperature and fasting was found due to high individual differences. (C) The scatter plot overlaying the bar charts shows the individual values for B. marinus liver. Values are means of four animals per group; *P<0.05, two-way ANOVA.





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