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Fig. 4. Effect of fasting on proton leak kinetics of isolated liver and skeletal
muscle mitochondria of WA (A) and CA (B) cane toads. Fasting revealed two
different mechanisms of decreasing the proton leak in liver mitochondria
(fasted toads, grey circles; WA fed toads, filled circles; CA fed toads, open
circles), dependent on acclimation temperature. In WA toads (A), fasting
caused a shift of the proton leak curve to the right, with no change in state
4 respiration but increased state 4 potential, thus suggesting a decrease in
proton conductance (similar to Fig.
3; P<0.05 comparing state 4 membrane potential). In
contrast, fasting in CA cane toads (B) resulted not only in a significant
decrease of membrane potential (P<0.05) but also in a strong
tendency towards lower state 4 respiration rates, suggesting a decreased
respiratory chain activity. In skeletal muscle mitochondria, fasting has no
effect on proton leakage at either acclimation temperatures (fasted toads,
grey circles; WA fed toads, filled squares;, CA fed toads, open squares).
Values are means ± s.e.m., N=8 for CA toads and N=9
for WA toads.
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