Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Whole animal standard metabolic rate (SMR) in ml O² h⁻¹ of fed and fasted cane toads acclimated to either 30°C (WA) or 10°C (CA), measured at their respective acclimation temperature. Values are means ± s.e.m., N=9 for warm acclimated and N=8 for cold acclimated toads. *P<0.05 (two-way ANOVA).

• Supplemental Figure S2 -

  Fig. S2. State 4 respiration rates of the four physiological conditions. The respiration rate of the WA fed group was set as 100% and all the other conditions were expressed relative to the WA fedstate. In the cold-acclimated toads, state 4 respiration tended to be decreased in response to fasting, indicating a lower activity of the respiratory chain. Values are means ± s.e.m. of 8 (CA) or 9 (WA) independent preparations.

• Supplemental Figure S3 -

  Fig. S3. Schematic (left) and physical gene maps (right) illustrate the conserved syntenic regions of the UCP1, 2 and 3 genes in the genomes of the human (Homo sapiens), mouse (Mus musculus), zebrafish (Danio rerio) and African clawed-frog (Xenopus tropicalis). Genes are shown as boxes and the horizontal arrows indicate the orientation of the gene. The protein identity of UCP neighboring genes is calculated as a percentage in respect to their human orthologs. (A) Conserved synteny at the UCP1 locus. Orthologs of Elmod2 could be identified in H. sapiens (GenBank accession number Q8IZ81), M. musculus (Ensembl gene ID ENSMUSG00000035151), D. rerio (GenBank accession number Q0VFW2), and X. tropicalis (Ensembl gene ID. ENSXETG00000014925), and orthologs of Gene A could be identified in H. sapiens (GenBank accession number NP_055945.2), M. musculus (Ensembl gene ID. ENSMUSG00000074228), D. rerio (Genbank accession number A2AW07), and X. tropicalis (GenBank accession number NP_055945). (B) Conserved synteny at the Ucp2-UCP3 locus. Orthologs of Dnajb13 are found in H. sapiens (Ensembl gene ID. ENSG00000187726), M. musculus (Ensembl gene ID. ENSMUSG00000030708), D. rerio (Ensembl gene ID. ENSDARG00000043157) and X. tropicalis (Ensembl gene ID. ENSXETG00000012541); Gene B neighboring UCP2-UCP3 are found in H. sapiens (GenBank accession number NP_056346.2), M. musculus (GenBank Acc. No. AU020772) and X. tropicalis (GenBank accession number NP_056346.2). The schemes on the right show the physical gene distances referring to the Ensembl genome browser (www.ensembl.org). Chr, chromosome; Dnajb13, DnaJ (Hsp40) related, subfamily B, member 13; Elmod2, ELMO domain-containing protein 2; PPME1, protein phosphatase methylesterase 1.