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Figure 1


Fig. 1. Vestibular epithelia, zones and afferent classes in the rodent. Confocal images have been combined to produce this picture of part of the vestibular portion of the mouse inner ear. The tissue is stained with fluorescently labeled phalloidin (yellow; labels hair bundles), as well as antisera to calretinin (green; stains certain afferents and hair cells) and β-III tubulin (red; stains afferents). Extracellular matrices have been removed to expose the hair cells in three sensory epithelia: the cristae of the anterior and horizontal semicircular canals and the macula of the utricle. The central zones of the cristae and the striolar zone of the utricular macula are outlined in red. At the bottom edge of the tissue is part of the vestibular ganglion. The large ganglion somata that are brightly stained for calretinin give rise to pure-calyx afferents to the central and striolar zones. Scale bar, 50 µm.





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