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Figure 4


Fig. 4. Involvement of the mitochondrial electron transport chain and potassium channels in embryonic rotation. (A) Rotenone (10 µmol l–1), an inhibitor of the mitochondrial electron transport chain, stimulated embryonic rotation (r.p.m., rotations per minute) after 10 min of incubation (left panel, N=15 embryos), but had no direct effect on the ciliary beat frequency (CBF) of isolated ciliary cells (right panel, N=5 cells). (B) 4-Aminopyridine (4-AP, 5 mmol l–1), a potassium channel inhibitor, induced prolonged increases in embryonic rotation (left panel, N=15 embryos), but had no direct effect on the CBF of isolated ciliary cells (right panel, N=5 cells). *P<0.05 compared with zero time point, ANOVA followed by Fisher's PLSD.





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