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Fig. 1. Morphological comparison of egg masses and embryos from H.
trivolvis (stage E25, top) and L. stagnalis (stage E39, bottom).
Whole egg masses and decapsulated embryos (inset) were imaged 3 days after egg
masses were laid by young adult snails. The characteristic tight planar
arrangement of egg capsules (open arrow) inside the egg mass (arrowhead) and
the flatter translucent embryo (arrow, also see inset) facilitate imaging of
early development and behaviour in H. trivolvis. The L.
stagnalis egg mass shown is considerably smaller than egg masses
typically laid by older snails, whereas the H. trivolvis egg mass is
more representative of the typical size. In the inset, embryos are shown in
left-side view under differential interference contrast (DIC) optics. The
stages shown represent the period of robust rotational behaviour for each
species. The thin scale bar represents 500 µm and the thick scale bar in
the inset represents 50 µm.
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