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Fig. 2. Relatively few neurons co-express Gyc-89Da and Gyc-89Db.
Flies were crossed to generate animals containing p89Da-GFP,
p89Db-GAL4 and UAS-dsRed. Each panel shows a confocal
z-stack, collapsed into a single plane with the red color
representing cells expressing Gyc-89Da and the green color
representing cells expressing Gyc-89Db. (A–D) Third instar
larvae. (E) Adult. (A) Dorsal view (anterior is up) of the terminal organ
(TO), terminal ganglion (TG), dorsal organ (DO) and dorsal ganglion (DG). Both
ganglia contain cells that express either Gyc-89Da or
Gyc-89Db but no cell shows co-expression of the two subunit genes.
Also shown are two neurons that are part of the monoscolopidial organ (mc)
that only express Gyc-89Db. (B) Lateral body wall showing the third
thoracic (T3) and first two abdominal segments (A1 and A2). In T3 two neurons
express both Gyc-89Da and Gyc-89Db (asterisks): a single
`les' neuron (upper) and a single `ves' neuron (lower). In A1 and A2 a single
`td' neuron expresses only Gyc-89Db (filled arrowheads). The fine red
lines are trachea closely associated with fine branches from the two td
neurons (open arrowheads). (C) Terminal segment showing the caudal sensory
cones (SO1–SO7). Each cone contains a single neuron that co-expresses
Gyc-89Da and Gyc-89Db. The inset shows a single neuron
extending a neurite to a cuticular peg sensillum (p). (D) Larval CNS showing
extensive expression of each subunit but no overlap in brain lobes (bl) and
ventral nerve cord (vnc). Expression is also seen in the ring gland (rg). (E)
Adult brain also shows extensive, non-overlapping expression of
Gyc-89da and Gyc-89Db. A, anterior; P, posterior; e, eyes.
Fluorescence seen in tracheae is due to autofluorescence. Scale bars represent
40 µm (A), 50 µm (C), 100 µm (B,D,E) and 25 µm (C inset).
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