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Figure 2


Fig. 2. Immunofluorescence staining of (A,B) Na+,K+-ATPase(green) and (A,C) occludin (red) in cross-sections of goldfish intestine. D is a merged image of B and C. In A, immunostaining for Na+,K+-ATPase is concentrated to basolateral regions and occludin immunostaining is apparent in apical regions of epithelia lining the intestinal lumen (L). Occludin immunostaining appears less prominent in epithelial cells at the base of the intestinal villi (see white arrows in A). At higher magnification (see dashed box in A, which outlines area magnified in C,D), occludin immunostaining appears as a typical honeycomb-like tight junction (TJ) pattern. Nuclei are stained with DAPI (blue), and only DAPI staining is observed in sections probed with secondary antibody alone (E). All scale bars are 20 µm.





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