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First published online December 14, 2007
Journal of Experimental Biology 211, 42-48 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.011882

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Differential actions of diuretic factors on the Malpighian tubules of Rhodnius prolixus

Andrew Donini^1,*, Michael J. O'Donnell² and Ian Orchard³

¹ Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada, M3J 1P3
² Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada, L8S 4K1
³ Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, Canada, L5L 1C6

^* Author for correspondence (e-mail: adonini{at}yorku.ca)

Accepted 22 October 2007

	Summary

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

 
The effects of corticotropin-releasing factor (CRF)-related (ZooneDH), calcitonin (CT)-related (RhoprDH₃₁) and kinin-related (leucokinin I) peptides on the ion composition of fluid secreted by upper Rhodnius prolixus Malpighian tubules and on KCl reabsorption by the lower tubules were assessed. ZooneDH stimulated fluid secretion while increasing the [Na⁺] of secreted fluid at the expense of [K⁺]. Upper tubules responded to ZooneDH with a characteristic triphasic change in the transepithelial potential (TEP), reminiscent of the response to 5-hydroxytryptamine (5HT). RhoprDH₃₁ produced a small (9 mV) lumen-positive shift in TEP of the upper tubule but had no effect on the rate of fluid secretion or ion composition of the secreted fluid. In contrast to 5HT, both peptides failed to activate KCl reabsorption by the lower tubule. Leucokinin I had no effect on the ion composition of fluid secreted by whole or upper Malpighian tubules. We propose that: (1) 5HT and a native CRF-related peptide similar to ZooneDH activate the same second messenger systems and ion transporters in the upper tubule cells; (2) CRF-related peptide is utilized to maintain high rates of fluid secretion during the post-feeding diuresis and is additionally used at times when KCl reabsorption is unnecessary or detrimental. The differential actions of multiple diuretic factors allows for intricate control of ionic and osmotic balance in R. prolixus.

Key words: Rhodnius, diuretic hormone, Malpighian tubules, transepithelial potential, secreted fluid, CRF-related peptide, kinin, DH31

	INTRODUCTION

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

 
Rhodnius prolixus periodically ingests a blood meal equivalent to 10 times its unfed weight. At these times, a rapid diuresis is initiated and the insect eliminates a large portion of the water and NaCl associated with the blood meal, while preserving its ionic and osmotic balance. The diuresis is controlled by the timely release of the hormone 5-hydroxytryptamine (5HT) and at least one neuropeptide, which is believed to also be a diuretic hormone (DH) (Lange et al., 1989; Maddrell et al., 1991; Maddrell, 1963). Both hormones stimulate rapid fluid secretion by the upper secretory segment of the Malpighian tubules (Maddrell, 1963; Maddrell et al., 1991). Stimulation by 5HT produces a characteristic triphasic change in transepithelial potential (TEP), corresponding to sequential activation of an apical Cl^– conductance (phase 1), an apical V-type H⁺-ATPase (phase 2) and a basal Na⁺–K⁺–2Cl^– cotransporter (phase 3) (O'Donnell and Maddrell, 1984; Ianowski and O'Donnell, 2001). The resulting movement of Na⁺, K⁺ and Cl^– from the hemolymph, across the tubule cells and into the tubule lumen drives the flow of osmotically obliged water through aquaporin-like channels (Echevarria et al., 2001; Martini et al., 2004). The actions of 5HT on the upper tubule are mediated by the second messenger cyclic AMP (Maddrell et al., 1971; Montoreano et al., 1990; Martini et al., 2004). Depletion of K⁺ from the hemolymph during this rapid diuresis is prevented by KCl reabsorbtion by the tubule's lower segment, through processes that are also stimulated by 5HT (Maddrell, 1978; Maddrell et al., 1993; Haley and O'Donnell, 1997). The effect of the peptidergic DH on the fluid secretion rates of upper tubules is similar to that of 5HT, but studies on the exact mechanisms of its actions have been hindered because the hormone has yet to be identified.

A number of peptides with diuretic effects have been discovered in other insects. These peptides belong to one of at least three families of peptides; the corticotropin-releasing factor (CRF)-related DHs, the calcitonin (CT)-related DHs and the kinin-related DHs. The CRF- and CT-related DHs act through cyclic AMP in tubules of several species to activate various transporters like the V-type H⁺-ATPase, Na⁺ channels and the Na⁺–K⁺–2Cl^– cotransporter (Plawner et al., 1991; Coast et al., 2005; Coast et al., 2002; Cabrero et al., 2002; Coast et al., 2001). The kinin-related DHs generally act through an increase in intracellular Ca²⁺ levels to activate Cl^– conductance pathways (O'Donnell et al., 1998; Beyenbach, 2003). The presence and release into the hemolymph of CRF-related, CT-related and kinin-related peptides in R. prolixus have been suggested on the basis of immunohistochemical, radioimmunological and high performance liquid chromatographic techniques (Te Brugge et al., 1999; Te Brugge and Orchard, 2002; Te Brugge et al., 2005). The exogenous CRF-related peptides ZooneDH and DippuDH₄₆ from Zootermopsis nevadensis and Diploptera punctata, respectively, stimulate fluid secretion and increase the cyclic AMP content of the upper tubules of R. prolixus (Te Brugge et al., 2002). The CRF-related peptides are potent stimulators of fluid secretion, capable of eliciting maximal rates of secretion comparable to those of 5HT-stimulated tubules (Te Brugge et al., 2002). In contrast, the CT-related peptide DippuDH₃₁ produces only small increases in secretion rates of R. prolixus upper tubules, equivalent to 1.5% of maximal rates achieved with 5HT (Te Brugge et al., 2005). Interestingly, the CT-related peptide of R. prolixus has an identical sequence to that of DippuDH₃₁ and is herein referred to as RhoprDH₃₁ (Te Brugge et al., in press). The non-native kinin-related peptides leucokinin I, leucokinin VIII and locustakinin all fail to increase the rate of fluid secretion in R. prolixus tubules (Te Brugge et al., 2002).

To date, no studies have been performed on the effects of CRF-related, CT-related and kinin-related peptides on TEP or the ion composition of the fluid secreted by the upper tubules. Furthermore, it is not known whether these factors activate KCl reabsorption by the lower tubule. The present study reports the K⁺, Na⁺ and Cl^– concentrations of the secreted fluid from upper tubules before and after treatment with 5HT, ZooneDH, RhoprDH₃₁ and leucokinin I. The results demonstrate that the actions of ZooneDH on the upper tubules are similar to those of 5HT. In addition, none of the peptides were capable of activating KCl reabsorption by the lower tubule.

	MATERIALS AND METHODS

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

 
Animals
Individuals of Rhodnius prolixus (Stål 1859) were obtained from a laboratory colony fed on rabbit blood and housed at 25°C under high humidity at the University of Toronto. Studies were conducted on unfed 5th instar larvae, 6–8 weeks post-emergence and that had previously fed on rabbit's blood as 4th instars. Experiments were conducted at room temperature (20°C).

Upper Malpighian tubule secretion assay
Insects were dissected under saline that contained (mmol l^–1): 129 NaCl, 8.6 KCl, 10.2 NaHCO₃, 4.3 NaH₂PO₄, 8.5 MgCl₂, 2 CaCl₂, 8.6 Hepes and 20 glucose at pH 7. Fine glass probes were used to dissect individual whole Malpighian tubules from the insects and the tubules were cut at the junction of the upper and lower segments. Individual upper segments of the tubules were transferred into 90 µl droplets of saline in a Sylgard-lined dish held under water-saturated paraffin oil. The open end of each tubule was pulled out of the saline droplet and wrapped around a minuten pin stuck into the Sylgard. Tubules were initially stimulated by adding a final concentration of 25 nmol l^–1 5HT to the saline droplets since unstimulated tubules secrete at very low rates of about 0.1 nl min^–1. ZooneDH, RhoprDH₃₁ and leucokinin I were subsequently added to the saline droplet at the final concentrations noted in the Results. Either for comparison purposes or to serve as a positive control, tubules in some experiments were treated with 1 µmol l^–1 5HT. Droplets of secreted fluid that formed at the pin were collected at intervals using fine glass probes. Fluid secretion rates were determined by measuring the diameter of the secreted fluid droplets with an ocular micrometer, calculating their volumes as (d³)/6, where d is the diameter, and dividing the volume by the time over which the droplets formed. The Na⁺, K⁺ and Cl^– concentrations of secreted fluid droplets were measured using ion-selective microelectrodes, as described below.

Whole Malpighian tubule secretion assay
The procedure for the dissection of whole tubules was the same as that described for the upper Malpighian tubules with the exception that the terminal ampulla was included and the tubules were not cut at the junction of the upper and lower segments. Whole tubules were transferred to saline droplets held under water-saturated paraffin oil. Each tubule was arranged such that the upper segment was held in a 90 µl droplet of saline modified to contain 24 mmol l^–1 KCl and 113.6 mmol l^–1 NaCl (all other solutes unaltered) and the lower segment was held in a separate 90 µl droplet of saline containing 4 mmol l^–1 KCl and 133.6 mmol l^–1 NaCl. This approach has been shown to maintain high rates of fluid and K⁺ secretion by the upper tubule and high rates of KCl reabsorption by the lower tubule (Haley and O'Donnell, 1997; Maddrell et al., 1993). The junction of the upper and lower segments was positioned in the paraffin oil between the two saline droplets. The ampulla was pulled out of the droplet holding the lower segment of the tubule and wrapped around a minuten pin. The upper segments of the tubules were stimulated by 1 µmol l^–1 5HT. Droplets of secreted fluid that had passed through the lower segment and emerged at the ampulla were collected for 10–15 min. This was followed by the addition of 1 µmol l^–1 ZooneDH, 0.1 µmol l^–1 RhoprDH₃₁ or 0.1 µmol l^–1 leucokinin I, the same concentrations of all three peptides at once, or 1 mmol l^–1 8-bromo-cyclic AMP to the saline droplet containing the lower segment of the tubule, and collection of fluid for a further 20–30 min. 5HT (1 µmol l^–1) was then added to the droplet bathing the lower tubule and fluid was collected for 10–30 min. Lastly, the tubule was cut at the junction between the upper and lower segments and fluid emerging from the upper segment was collected. Ion concentrations in the collected droplets were measured using ion-selective microelectrodes.

Measurement of the transepithelial potential of upper Malpighian tubules
A previous study has validated the use of the Ramsay technique for the measurement of TEP in upper Malpighian tubules of R. prolixus (Ianowski and O'Donnell, 2001). Isolated upper tubules from 5th instars were transferred to saline droplets under paraffin oil. The cut end of the tubule was pulled out of the droplet and wrapped around a minuten pin. A fine glass probe was used to make a small hole in the tubule wall between the saline droplet and the minuten pin. The tip of a microelectrode filled with 3 mol l^–1 KCl was placed in the secreted fluid that emerged from the hole in the tubule while a second, similar microelectrode was placed in the saline droplet. The electrodes were connected through a high impedance (10¹³ ) ML165 pH Amp to a Powerlab 4/30 data acquisition system (ADInstruments, Colorado Springs, CO, USA). ZooneDH (1 µmol l^–1) or RhoprDH₃₁ (0.1 µmol l^–1) was added to the saline droplet while recording the transepithelial potential.

Construction of ion-selective microelectrodes
Microelectrodes were fabricated as described previously (Rheault and O'Donnell, 2001; Donini and O'Donnell, 2005). The following ionophore cocktails and backfill solutions (in parentheses) were used: Na⁺ ionophore II cocktail A (500 mmol l^–1 NaCl), K⁺ ionophore I cocktail B (500 mmol l^–1 KCl). The tips of these electrodes were dipped in a solution of polyvinylchloride (PVC, Fluka, Buchs, Switzerland) in tetrahydrofuran (THF, Fluka) to permit their use in fluid droplets under paraffin oil (see Rheault and O'Donnell, 2004). For the measurement of Cl^–, a solid state silver/silver chloride microelectrode was employed as previously described (Donini and O'Donnell, 2005). The microelectrodes were calibrated in the following solutions: Na⁺, 15 mmol l^–1 NaCl/135 mmol ^–1 LiCl and 150 mmol l^–1 NaCl; K⁺, 15 mmol l^–1 KCl/135 mmol l^–1 NaCl and 150 mmol l^–1 KCl; Cl^–, 20 mmol l^–1 KCl and 200 mmol l^–1 KCl. Slopes for the electrodes [mean ± s.e.m. (N)] for a tenfold change in ion concentration were 53.9±1.4 mV (9) for Na⁺, 55.1±0.5 mV (26) for K⁺ and 58.2±2 mV (9) for Cl^–. The reference microelectrode was filled with a solution of 500 mmol l^–1 KCl. Electrodes were connected to a data acquisition system as described for the measurement of transepithelial potential. The ion concentration of the secreted fluid was calculated using the following formula: [ion]_sf=Cx10^(V/slope), where [ion]_sf is the ion concentration in the secreted fluid droplet; C is the ion concentration in one of the calibration solutions used to calibrate the electrodes; V is the voltage difference between the droplet of secreted fluid and the same calibration solution; and the slope is the change in voltage measured by the electrode in response to a tenfold change in ion concentration. K⁺ interferes with the response of the Na⁺ electrode, and the Na⁺ concentrations were therefore corrected using the Nicolsky–Eisenman equation (Ammann, 1986) and a selectivity coefficient (K_NaK) of 10^0.32. Although ion-selective microelectrodes measure ion activity and not concentration, data can be expressed in terms of concentration if it is assumed that the ion activity coefficient is the same in calibration and experimental solutions.

View larger version (18K): [in this window] [in a new window]   Fig. 1. Upper tubules were initially stimulated with 25 nmol l–1 5-hydroxytryptamine (5HT) in saline and fluid was collected at 10 min intervals for a total of 20 min. The tubules then received 1 µmol l–1 of either 5HT (filled squares) or ZooneDH (filled triangles) and fluid was collected for a further 55 min at 5 min intervals. The rate of fluid secretion (A) was calculated after measuring the diameter of each droplet, and ion-selective microelectrodes were used to measure the K+ (B), Na+ (C) and Cl– (D) concentration of each droplet of secreted fluid. ZooneDH and 5HT stimulate fluid secretion to a similar extent and both increase the Na+ concentration of the secreted fluid at the expense of K+ over time. Each point is the mean ± s.e.m. of 6–9 individual tubules.

	RESULTS

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

Addition of 5HT (1 µmol l^–1) produced a rapid increase in the rate of fluid secretion, which peaked within 15 min (Fig. 1A). ZooneDH (1 µmol l^–1) produced a similar increase in the rate of fluid secretion, although the response was slower, peaking at 25 min after applying ZooneDH.

Both 5HT and ZooneDH (1 µmol l^–1) caused an increase in the Na⁺ concentration of the secreted fluid at the expense of K⁺ over time (Fig. 1B,C). The Na⁺ and K⁺ concentration of the secreted fluid changed by approximately 20–30 mmol l^–1. The [K⁺]/[Na⁺] ratios 55 min after the addition of 1 µmol l^–1 5HT or ZooneDH were 0.41±0.03 and 0.44±0.04, respectively. These values are significantly lower than those in response to 25 nmol l^–1 5HT alone (Student's t-test, P=0.003 and P=0.001 for 5HT and ZooneDH, respectively). Neither 5HT nor ZooneDH had significant effects on the Cl^– concentration of the secreted fluid over time (Fig. 1D).

R. prolixus upper tubules are sensitive to changes in the bath concentration of K⁺, responding to a decrease in K⁺ bath concentration with a decrease in the overall secretion of K⁺ (Maddrell et al., 1993). However, our results do not reflect K⁺ depletion of the bathing saline resulting from the high rates of ion transport by the tubules. The final [K⁺] in the bathing droplet at the end of the experiments (9.78±0.7 mmol l^–1) was not different from that at the beginning (8.45±0.3 mmol l^–1) for tubules stimulated with 5HT. Corresponding values for tubules stimulated with ZooneDH were 8.1±0.6 and 9.1±0.2 mmol l^–1, respectively.

A high concentration (0.1 µmol l^–1) of RhoprDH₃₁ alone increases fluid secretion by upper tubules to 1.5% of the maximal value seen in response to 1 µmol l^–1 5HT (Te Brugge et al., 2005). There is no effect of leucokinin I (0.1 µmol l^–1) on fluid secretion rate (Te Brugge et al., 2005). In the presence of a low concentration of 5HT (25 nmol l^–1), neither RhoprDH₃₁ nor leucokinin I (0.1 µmol l^–1) altered fluid secretion rate or the composition of the secreted fluid (Fig. 2). These tubules secreted at high rates upon the addition of 1 µmol l^–1 5HT over the final 15 min of the experiment and without altering the [K⁺]/[Na⁺] ratio.

View larger version (18K): [in this window] [in a new window]   Fig. 2. Upper tubules were initially stimulated with 25 nmol l–1 5HT in saline and fluid was collected at 10 min intervals for a total of 30 min. The tubules were then maintained in 25 nmol l–1 5HT (filled triangles) or received 0.1 µmol l–1 of either RhoprDH31 (filled circles) or leucokinin I (filled squares) at the solid arrow, and fluid was collected for a further 40 min at 10 min intervals. The rate of fluid secretion (A) was calculated after measuring the diameter of each droplet, and ion-selective microelectrodes were used to measure the K+ (B), Na+ (C) and Cl– (D) concentration of each droplet of secreted fluid. RhoprDH31 and leucokinin I had no effect on the rate of fluid secretion or the ion composition of the secreted fluid. Each point is the mean ± s.e.m. of 7–9 individual tubules. Addition of 1 µmol l–1 5HT at the dashed arrow was used to confirm the viability of the Malpighian tubules.

View larger version (11K): [in this window] [in a new window]   Fig. 3. Transepithelial potential (TEP) of the upper, secretory segment of the Malpighian tubules. (A) Representative recording showing the effects of 1 µmol l–1 ZooneDH (arrow). Three distinct phases of the response are numbered on the trace. The dotted line indicates a potential of 0 mV, when both microelectrodes were placed in the bathing droplet. (B) The mean ± s.e.m. (N=5) TEP prior to the addition of ZooneDH (Rest) and during the three phases of the response. (C) Representative recording showing the effects of 0.1 µmol l–1 RhoprDH31 (solid arrow) and 1 µmol l–1 5HT (dashed arrow). The three phases of the TEP response to 5HT are numbered. (D) The mean ± s.e.m. (N=5) TEP prior to the addition of RhoprDH31 (Rest), 10 min after the addition of RhoprDH31 (DH31), and during the three phases of the 5HT response. Asterisks denote a significant difference from the resting TEP (ANOVA, followed by Tukey–Kramer).

A high concentration of RhoprDH₃₁ resulted in a positive deflection of the TEP from the unstimulated value of –34.8±3.8 mV to –25±3.4 mV (N=5), which stabilized within 5 min. These tubules subsequently responded to a high concentration of 5HT in the characteristic triphasic manner with phase 1 consisting of a slight (2 mV) negative deflection, phase 2 a large (60 mV) positive deflection and phase 3 returning to –15±5.3 mV, almost 20 mV more positive than the TEP prior to the addition of 5HT.

View larger version (26K): [in this window] [in a new window]   Fig. 4. Whole Malpighian tubules were removed from animals and a modified Ramsay secretion assay employing two separate bathing droplets for the upper and lower segments of the tubules was used to collect secreted fluid. Ion-selective microelectrodes were used to measure the K+ and Cl– concentration of the secreted fluid. Upper tubules were stimulated with 1 µmol l–1 5HT and the lower tubules received 1 µmol l–1 ZooneDH (A), 0.1 µmol l–1 RhoprDH31 (B), 0.1 µmol l–1 leucokinin I (C,D), all three peptides at once (E) or 1 mmol l–1 8-bromo-cyclic AMP (F; solid arrow in A–F). In addition, the lower tubules subsequently received 1 µmol l–1 5HT (dashed arrow in A–F) as a positive control. A sample of the fluid from the upper tubule was taken at the end of the experiment by cutting the tubule at the junction of the upper and lower tubule (UMT). Values are means ± s.e.m. for A: N=4; B: N=5; C and D: N=6–11; E: N=7; F: N=6.

	DISCUSSION

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

 
This is the first study to examine the actions of CRF-related, CT-related and kinin-related peptides on the ion composition of secreted fluid and/or on transepithelial potentials of Malpighian tubules of R. prolixus. The CRF-related peptides ZooneDH, DippuDH₄₆ and LocustaDH were previously shown to stimulate the fluid secretion of R. prolixus Malpighian tubules at rates similar to those elicited by high concentrations of 5HT (Te Brugge et al., 2002; Coast, 1997). Upon application of ZooneDH to upper segments of the tubules and in the presence of a low concentration of 5HT, the Na⁺ concentration of the secreted fluid increased at the expense of K⁺. This effect was similar to the effect of a high concentration of 5HT on the tubules. In both cases the [K⁺]/[Na⁺] ratio was significantly reduced over time. The resulting K⁺ and Na⁺ concentrations of the secreted fluid collected 55 min after application of 1 µmol l^–1 5HT were 52±3 and 132±5.8 mmol l^–1, respectively. The K⁺ concentration is slightly lower than the 70 mmol l^–1 previously reported by Maddrell and Phillips (Maddrell and Phillips, 1975), whereas the Na⁺ concentration is similar to the value of 125 mmol l^–1 reported in the same study. The apparent natriuretic activity of ZooneDH is consistent with another CRF-related and native peptide, Achdo-DH, in the cricket (Coast et al., 2002); however, another CRF-related peptide in the mosquito, Anoga-DH₄₄, does not possess natriuretic activity (Coast et al., 2005). All these peptides, as well as 5HT in R. prolixus, appear to act through cyclic AMP.

Detailed comparison of the changes in transepithelial potential in response to ZooneDH and 5HT reveals that the magnitude and timing of each of the three phases is approximately the same. Pharmacological studies have attributed each phase of the 5HT response to a specific ion-transport mechanism in the apical or basal membranes of the upper tubule cells (Ianowski and O'Donnell, 2001). It therefore seems likely that ZooneDH affects the same ion transporters as 5HT. Our results, coupled with previous findings that ZooneDH and 5HT both increase intracellular cyclic AMP levels (see Te Brugge et al., 1999; Te Brugge et al., 2002) in the upper tubule cells, strongly suggest that a native CRF-related peptide and 5HT in R. prolixus activate the same second messenger pathways and ion-transport mechanisms in the upper tubule cells.

In our studies, the native CT-related peptide RhoprDH₃₁ had no significant effect on the rate of fluid secretion or the ion composition of the secreted fluid when it was applied in the presence of 25 nmol l^–1 5HT. Previous reports showed a significant increase in the rate of fluid secretion when the peptide (referred to then as Dippu-DH₃₁) was applied alone; however, the maximal rates achieved were only 1.5% of those obtained with a high concentration of 5HT, and the actions of the peptide were highly variable (Te Brugge et al., 2005). Given the small lumen-positive change in TEP in response to RhoprDH₃₁, the peptide may stimulate the V-type H⁺-ATPase on the apical membrane (i.e. phase 2 of the response to 5HT or ZooneDH). If this is the case, then it would seem that the effects of RhoprDH₃₁ on the proton pump are relatively small (when compared with ZooneDH or 5HT). Co-localisation of 5HT and RhoprDH₃₁-like material is found in cell bodies and their neurohemal sites on the abdominal nerves, suggesting that these two factors may be co-released (Te Brugge et al., 2005). When 1 µmol l^–1 5HT is applied in the presence of RhoprDH₃₁, phase 1 of the 5HT response, which is proposed to correspond to activation of an apical Cl^– conductance, is reduced [2 mV shift, see Fig. 3, and 10 mV shift with 5HT alone (see Ianowski and O'Donnell, 2001)]. This pattern may reflect partial stimulation of proton pump activity by RhoprDH₃₁ and a consequent lumen-positive shift in TEP, so that the lumen-negative shift produced by the increase in Cl^– conductance during phase 1 of the 5HT response is reduced. Phase 2 of the 5HT response is no different in the presence of RhoprDH₃₁ (60 mV shift from phase 1), which is consistent with a maximal stimulation of the proton pump. Phase 3 of the 5HT response, corresponding to the subsequent activation of the basal Na⁺–K⁺–2Cl^– cotransporter, in the presence of RhoprDH₃₁ resulted in a TEP which was 20 mV more positive than the TEP prior to the addition of 5HT. In contrast, 5HT alone results in a phase 3 TEP, which is only 10 mV more positive than the resting TEP (see Ianowski and O'Donnell, 2001) (Fig. 2A). This result is also consistent with a continued potentiation of proton pump activity by RhoprDH₃₁.

In contrast to the secretory nature of the upper tubules, the lowermost segments of the tubules are responsible for the reabsorption of KCl (Maddrell and Phillips, 1975). Reabsorption is activated by 5HT and the cells of the lower tubules respond more rapidly to 5HT than those of the upper tubule (Maddrell et al., 1993). This is a particularly important physiological role since the high rate of KCl transport from hemolymph to lumen by the upper tubules would quickly deplete the hemolymph of K⁺ without reabsorption of K⁺ downstream. Our results demonstrate that ZooneDH and RhoprDH₃₁ cannot activate KCl reabsorption by the lower tubules. Interestingly, Maddrell (Maddrell, 1976) suggested that different regions of the tubule are not controlled by a single hormone since extracts of lateral neurosecretory cells of the mesothoracic ganglionic mass stimulated secretion from the upper tubules but did not stimulate KCl reabsorption from the lower tubule. The membrane-permeable and phosphodiesterase-resistant cyclic AMP analog 8-bromo-cyclic AMP at least partially activates K⁺ reabsorption, suggesting that cyclic AMP may act as a second messenger for activation of one or more of the KCl transporters in the lower tubule. Moreover, since 5HT, CRF-related and CT-related peptides all appear to make use of cyclic AMP as a second messenger, our results suggest that the cells of the lower tubules may not express receptors for CRF-related and CT-related peptides.

Leucokinin I had no measurable effect on the Malpighian tubules of R. prolixus. Te Brugge et al. (Te Brugge et al., 2002) tested for effects of a number of kinin-like peptides on the upper tubules of R. prolixus, revealing that none of these peptides affected the rate of fluid secretion. Based on our results, these findings can be extended to include no effect on the ion composition of the secreted fluid or on the lower tubule. In addition, application of all three peptides at once to the lower tubule had no effect on K⁺ concentration of the secreted fluid from whole tubules.

In summary, this study demonstrates that various putative diuretic factors have different actions on the Malpighian tubules of R. prolixus. Although CRF-related factors and 5HT have similar, if not identical, actions on the upper tubules, an important difference is that CRF-related factors cannot activate KCl reabsorption by the lower tubule. The native CT-related factor RhoprDH₃₁ has minimal and variable effects on fluid secretion (Te Brugge et al., 2005) and produces a small (9 mV) increase in the TEP of upper tubules. Although our findings suggest that kinin-like factors do not act on upper or lower Malpighian tubules of R. prolixus, kinins may still be important in the post-feeding diuresis by acting on other relevant tissues such as the crop and hindgut (see Te Brugge et al., 2002).

It thus appears that R. prolixus has multiple signaling mechanisms that permit complex control of hemolymph ionic and osmotic balance. During the post-feeding diuresis, we propose that the quick and timely release of 5HT (see Lange et al., 1989) serves to activate KCl reabsorption by the lower tubule and rapid fluid secretion by the upper tubule, which may be potentiated by the co-release of RhoprDH₃₁ (see Maddrell et al., 1993; Te Brugge et al., 2005). When hemolymph titers of 5HT fall to levels that are insufficient to maintain high rates of fluid secretion by the upper tubules, release of a native CRF-related peptide can be utilized to maintain these high rates of secretion. In addition there are likely to be other times when stimulation of upper tubule ion transport is needed without reabsorption of KCl by the lower tubule. For instance, digestion of the red blood cells from the bloodmeal may lead to a K⁺ load, which the insect needs to eliminate. In this case the CRF-related peptide may be released in the absence of 5HT.

	References

TOP Summary INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION References

 

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