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Figure 4


Fig. 4. Cardiac myofibril cytoarchitecture in mlp84B-deficient flies. mpl84B–/– (A,B) and mlp84B–/– rescue (C,D) adult Drosophila hearts were co-stained with anti-{alpha}-actinin and anti-Mlp84B antibodies. mlp84B–/– hearts show sarcomeric disorganization of the longitudinal fibers as evidenced by misalignments and gaps in {alpha}-actinin-positive bands (A). Sarcomeric structure is restored in the mlp84B–/– rescue line (C). Cardiac spiral myofibers were visualized in cypher-GFP;mlp84B–/– (E,F) and cypher-GFP;mlp84B–/– rescue (G,H) hearts. The A3 segment is shown, including the ostia in the center. No obvious structural defect was detected (E). All scale bars are 20 µm.





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