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Figure 1


Fig. 1. Variability of the Root effect in haemolysates of selected African fishes. The magnitude of the Root effect is measured as the difference in percentage Hb O2 saturation between the two plateaus at high and low pH. Presence and absence of choroid and swimbladder retia mirabilia are indicated for each species. Hb O2 saturation of functional Hb was determined using diluted, air-equilibrated samples (5–15 µmol l–1 [Hb4]) by spectral deconvolution between 500 and 700 nm (Völkel and Berenbrink, 2000). Measurements were performed at 25°C without removal of organic phosphates in 50 mmol l–1 Tris HCl buffer (pH 8.0–6.5) and 50 mmol l–1 citrate buffer (pH 7.0–5.0) in the presence of 0.1 mol l–1 KCl (M. Berenbrink, unpublished). Fish line drawings modified after: Lehrbuch der Speziellen Zoologie. Begruendet von Alfred Kaestner. Band II: Wirbeltiere, 1991©Elsevier GmbH, Spektrum Akademischer Verlag, Heidelberg.





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