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Fig. 3. Presence of Takeout (TO) in the head fat body, which is hypertrophic in to¹. (A) Immunohistological staining using an anti-TO antibody. (Ai,Aii) TO is detected in the head fat body (arrows) of a wild-type Canton Special (CS) male, whereas in a to¹ mutant it is not detected (10x; scale bar, 100 µm). (Aiii,Aiv) In wild-type CS, TO is also detected in the gustatory sensilla (arrows) (Aiii: 20x; scale bar, 50 µm. Aiv: 40x; scale bar, 25 µm). (B) Hematoxylin-Eosin (HE) staining reveals that under normal feeding conditions, to flies exhibit a hypertrophic head fat body (outlined in red). The 40x field corresponds to the 10x outlined area. The head fat body cells in to¹ exhibit large lipid vacuoles (40x image). This phenotype is rescued by directed genetic expression of to within the to-producing cells. (40x: scale bar, 25 µm; 10x: scale bar, 100 µm). (C) After a 16-h period of starvation, the head fat body is reduced in wild-type flies, because they have mobilized their energy resource. The HE staining is more intense in the head fat body of wild-type flies as cells have shrunk and cell membranes are tightly regrouped. In to¹ flies, the fat body is also reduced, but less so than in wild type. This phenotype is also rescued by directed genetic expression of to within TO-producing cells. Rescue, tim-GAL4/UAS-to in a to¹ genetic background; to¹, to mutant.

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