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Fig. 2. Triglyceride and trehalose levels before and after starvation. (A) The
total amount of triglycerides was quantified for each genotype before and
after starvation. Prior to starvation, female and male to1
flies display an increase in the total amount of triglycerides. This phenotype
is fully rescued by directed genetic expression of takeout (rescue)
in both sexes. After 16 h starvation, the triglyceride level is reduced for
all genotypes but the amount of triglycerides remains higher for
to1. This phenotype was also rescued in males, but not in
females. (B) The total amount of trehalose was quantified for each genotype
before and after starvation. Female and male wild-types displayed a decrease
in their level of trehalose after starvation. However, starvation had no
effect on trehalose level in to1 flies. As for the
triglyceride level, this phenotype was rescued genetically. The total amount
of triglycerides and trehalose was determined on groups of 10 flies. The
number in each box represents the number of fly groups used for each genotype.
In B, for the statistical analysis, the comparison was performed, for the same
genotype, before and after starvation. CS, Canton Special; rescue,
tim-GAL4/UAS-to in a to1 genetic
background; to1, to mutant. *P<0.05;
**P<0.001; ***P<0.0001. Mean ± s.e.m.
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