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Figure 9


Fig. 9. The effect of heat shock on coelomocyte spreading. Isolated coelomocytes were heat-shocked in isotonic medium at 31°C for 30 min. Controls remained at room temperature. The percentages of cells on each slide that had petaloid, fibroblastic, or filopodial morphology were determined. Heat-shocked cells showed significantly more petaloid forms and fewer filopodial forms than unshocked or hypotonically shocked cells (asterisks, P<0.05). Thus, heat shock prior to hypotonic shock inhibited coelomocyte spreading to a similar extent as exogenous Hsp70 and Hsc70. Values are means ± s.e.m.





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