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Fig. 7. The effect of endotoxin on cell spreading in hypotonically shocked
coelomocytes. Recombinant proteins may be contaminated with small amounts of
bacterial endotoxin. To confirm that inhibition of coelomocyte spreading in
the presence of recombinant Hsps was not a result of endotoxin, hypotonically
shocked coelomocytes were exposed to endotoxin in concentrations similar to
those measured in the recombinant Hsps. The endotoxin-treated cells were
compared to cells hypotonically shocked (control) and hypotonically shocked in
the presence of Hsp70 or Hsc70. Endotoxin-treated cells were not significantly
different from control cells that had received only a hypotonic shock. The
Hsp70- and Hsc70-treated cells were significantly different from both the
endotoxin-treated and control cells, as indicated by the asterisk
(P<0.05). Values are means ± s.e.m. N=5 replicate
experiments in which at least 100 cells were observed.
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