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Fig. 5. Effect of Hsc70 on immunoreactive phospho-cdc2 levels following
fertilization. (A) Gray-scale image of one blot of immunoreactive
phospho-cdc2. (B) The same image in pseudocolor to emphasize the relative
differences in each lane. The color spectrum bar shows the grayscale-to-color
correspondence, with violet representing black, and red representing white.
Variation in background is what accounts for the blue color in the left half
of the blot and the blue-green on the right. Lanes 1 and 2 are unfertilized
eggs collected at 0 and 50 min after 5 µg ml–1 Hsc70.
Lanes 3 and 4 are fertilized eggs in 5 µg ml–1 Hsc70 at 25
and 50 min after fertilization, respectively. Lanes 5 and 6 are fertilized
eggs in the absence of Hsc70 at 35 and 50 min after fertilization,
respectively. Phospho-cdc2 remained at a higher level in Hsc70-treated eggs
for the first 25–35 min after fertilization (compare lanes 3 and 5), but
was about the same in the two groups by 50 min after fertilization. This blot
is one of two, each showing the same trends.
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